Generated:Article Title: Effects of different types of exercise training on angiogenic responses in the left ventricular muscle of aged rats.
Article Snippet: Background: We evaluated angiogenic responses in the left ventricular muscle and aerobic capacity according to exercise type (aerobic, resistance, combined) in aged rats.. Methods: In total, 24 male Sprague-Dawley rats (100 weeks old) were used.. To investigate the effect of regular training, the rats were divided into non-exercise (NE), aerobic exercise (AE), resistance exercise (RE), and combined exercise (CE) groups (six rats per group).
Marker:Article Title: Effects of different types of exercise training on angiogenic responses in the left ventricular muscle of aged rats.
Article Snippet: Background: We evaluated angiogenic responses in the left ventricular muscle and aerobic capacity according to exercise type (aerobic, resistance, combined) in aged rats.. Methods: In total, 24 male Sprague-Dawley rats (100 weeks old) were used.. To investigate the effect of regular training, the rats were divided into non-exercise (NE), aerobic exercise (AE), resistance exercise (RE), and combined exercise (CE) groups (six rats per group).
Article Title: Methods for treating alzheimer disease and for reducing amyloid beta formation
Article Snippet: .. Protein Size Marker (Bio-Rad) and each sample were added to Stacking Gel Well prepared in Mini-Protein II Dual-Slab Apparatus (Bio-Rad) and electrophoresed at 150 volt till all are precipitated to the bottom. ..
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson's disease.
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. Western blotting After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Methods for treating alzheimer disease and for reducing amyloid beta formation
Article Snippet: .. Protein Size Marker (Bio-Rad) and each sample was injected into Stacking Gel Well equipped in Mini-Protein II Dual-Slab Apparatus (Bio-Rad) and electrophoresed at 150 volt until all are settle at the bottom. ..
Molecular Weight:Article Title: Effects of different types of exercise training on angiogenic responses in the left ventricular muscle of aged rats.
Article Snippet: Background: We evaluated angiogenic responses in the left ventricular muscle and aerobic capacity according to exercise type (aerobic, resistance, combined) in aged rats.. Methods: In total, 24 male Sprague-Dawley rats (100 weeks old) were used.. To investigate the effect of regular training, the rats were divided into non-exercise (NE), aerobic exercise (AE), resistance exercise (RE), and combined exercise (CE) groups (six rats per group).
Concentration Assay:Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson's disease.
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. Western blotting After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Membrane:Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson's disease.
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. Western blotting After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Activation Assay:Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson's disease.
Article Snippet: .. After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. Western blotting After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Recombinant:Article Title: Glycylglycine promotes the solubility and antigenic utility of recombinant HCV structural proteins in a point-of-care immunoassay for detection of active viremia.
Article Snippet: Finally, the purified recombinant proteins were detoxified using PierceTM high capacity endotoxin removal spin columns (ThermoFisher Scientific, USA) according to the user manual. .. Recombinant protein preparations were resolved by SDS‒PAGE using a Bio-Rad Mini-Protein II Dual-Slab apparatus according to Laemmli [48] for detection of the molecular weights of recombinant proteins compared to the expected values based on the amino acid number in the amplified genes. .. To assess the antigenicity of recombinant proteins by their ability to recognize corresponding antibodies in patients’ sera, western blot analysis of protein samples was carried out in mini-transblot electrophoretic transfer cells (Bio-Rad-USA) according to Towbin et al. [49].
Article Title: Glycylglycine promotes the solubility and antigenic utility of recombinant HCV structural proteins in a point-of-care immunoassay for detection of active viremia
Article Snippet: Finally, the purified recombinant proteins were detoxified using PierceTM high capacity endotoxin removal spin columns (ThermoFisher Scientific, USA) according to the user manual. .. Recombinant protein preparations were resolved by SDS‒PAGE using a Bio-Rad Mini-Protein II Dual-Slab apparatus according to Laemmli [ ] for detection of the molecular weights of recombinant proteins compared to the expected values based on the amino acid number in the amplified genes. .. To assess the antigenicity of recombinant proteins by their ability to recognize corresponding antibodies in patients' sera, western blot analysis of protein samples was carried out in mini-transblot electrophoretic transfer cells (Bio-Rad-USA) according to Towbin et al. [ ].
Amplification:Article Title: Glycylglycine promotes the solubility and antigenic utility of recombinant HCV structural proteins in a point-of-care immunoassay for detection of active viremia.
Article Snippet: Finally, the purified recombinant proteins were detoxified using PierceTM high capacity endotoxin removal spin columns (ThermoFisher Scientific, USA) according to the user manual. .. Recombinant protein preparations were resolved by SDS‒PAGE using a Bio-Rad Mini-Protein II Dual-Slab apparatus according to Laemmli [48] for detection of the molecular weights of recombinant proteins compared to the expected values based on the amino acid number in the amplified genes. .. To assess the antigenicity of recombinant proteins by their ability to recognize corresponding antibodies in patients’ sera, western blot analysis of protein samples was carried out in mini-transblot electrophoretic transfer cells (Bio-Rad-USA) according to Towbin et al. [49].
Article Title: Glycylglycine promotes the solubility and antigenic utility of recombinant HCV structural proteins in a point-of-care immunoassay for detection of active viremia
Article Snippet: Finally, the purified recombinant proteins were detoxified using PierceTM high capacity endotoxin removal spin columns (ThermoFisher Scientific, USA) according to the user manual. .. Recombinant protein preparations were resolved by SDS‒PAGE using a Bio-Rad Mini-Protein II Dual-Slab apparatus according to Laemmli [ ] for detection of the molecular weights of recombinant proteins compared to the expected values based on the amino acid number in the amplified genes. .. To assess the antigenicity of recombinant proteins by their ability to recognize corresponding antibodies in patients' sera, western blot analysis of protein samples was carried out in mini-transblot electrophoretic transfer cells (Bio-Rad-USA) according to Towbin et al. [ ].
Western Blot:Article Title: Neuroprotective effect of treadmill exercise possibly via regulation of lysosomal degradation molecules in mice with pharmacologically induced Parkinson’s disease
Article Snippet: .. Western blotting After the protein sample was loaded into the stacking gel well in the Mini-Protein II dual-slab apparatus (Bio-Rad, CA, USA) with a standard marker (Page Rular Pre-stained Protein Ladder #SM0671-Fermertas) at a concentration of 30 μg total protein, the protein sample was electrophoresed at 80 V. A polyvinylidine difluoride (PVDF) membrane (Immuno-Blot, PVDF, Bio-Rad, CA, USA) was reacted to induce membrane activation for 2 min. Proteins were transferred for 1 h at a constant voltage of 200 mA. .. Each membrane was blocked in 3% BSA, followed by incubation with the primary antibody: α-synuclein (BD bioscience, 1:1000); tyrosine hydroxylase (Millipore, 1:1000); PINK1 (Abcam, 1:1000); parkin (Abcam, 1:1000); p62 (Cell Signaling, 1:1000); LC3A/B (Abcam, 1:1000); LAMP2 (Santa Cruz, 1:1000); cathepsin L (Abcam, 1:1000); Tom 20 (Santa Cruz, 1:1000); β-actin (Santa Cruz, 1:1000) diluted in 3% BSA for 12 h at 4 °C.
Injection:Article Title: Methods for treating alzheimer disease and for reducing amyloid beta formation
Article Snippet: .. Protein Size Marker (Bio-Rad) and each sample was injected into Stacking Gel Well equipped in Mini-Protein II Dual-Slab Apparatus (Bio-Rad) and electrophoresed at 150 volt until all are settle at the bottom. ..
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